Protein_Domain
Part:BBa_J70595:Design
Designed by: Joseph Lynch Group: Knight Lab (2010-06-23)
RFC12 Green Fluorescent Protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 641
Design Notes
Amplified via colony PCR directly from the BBa_E0040 cell stock. Note that both the ATG and double TAA codons were removed.
Forward Primer:
5' GTTTCTT C GAATTC GCGGCGC T ACTAGT CGTAAAGGAGAAGAACTTTTCACTG 3', GFP-F
Reverse Primer:
5' GTTTCTT C CTGCAG CGGCCGC T GCTAGC TTTGTATAGTTCATCCATGCCATGTG 3', GFP-R
Source
BBa_E0040